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duoset elisa kits  (R&D Systems)


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    R&D Systems duoset elisa kits
    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
    Duoset Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duoset+kit/Human+IL-8%2FCXCL8+DuoSet+ELISA/pmc13623111-201-9-12
    Average 96 stars, based on 647 article reviews
    duoset elisa kits - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Functional Heterogeneity of the Autism Spectrum Disorder‐Associated Gut Microbial Ecosystem Revealed by Fecal Lipopolysaccharides and Bacterial Proteomic Profiling"

    Article Title: Functional Heterogeneity of the Autism Spectrum Disorder‐Associated Gut Microbial Ecosystem Revealed by Fecal Lipopolysaccharides and Bacterial Proteomic Profiling

    Journal: Chembiochem

    doi: 10.1002/cbic.70557

    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
    Figure Legend Snippet: Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Techniques Used: Activation Assay, MTT Assay, Negative Control, Enzyme-linked Immunosorbent Assay, Comparison

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Guanylate-binding protein-5 is involved in inflammasome activation by bacterial DNA but only the cooperation of multiple GBPs accounts for control of Brucella abortus infection.
    Article Snippet: As a positive control for inflammasome activation, cells were primed with Pam (1 mg/mL) for 4 hours following addition of 20 mM nigericin sodium salt (Sigma-Aldrich) in the last 45 minutes of incubation. .. IL-1b, TNF-a, IL-6 and IL-12p40 production was measured in the supernatants of cells by ELISA using a DuoSet kit (R&D Systems, Minneapolis, MN), according to the manufacturer’s guidelines. ..

    Article Title: Effect of dietary supplementation of macadamia oil on the growth, immune function, physio-biochemical components and thyroid activity of growing rabbits.
    Article Snippet: Department of Biological Sciences, College of Science, University of Jeddah, Jeddah, Saudi Arabia Chemistry Department, College of Science, King Khalid University, Abha, Saudi Arabia Department of Food Science and Nutrition, Faculty of Home Science, University of Tabuk, Tabuk, Saudi Arabia Department of Biology, College of Science, Princess Nourah Bint Abdul Rahman University, Riyadh, Saudi Arabia Agricultural Research Center, Animal Production Research Institute, Dokki, Egypt Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Helwan, Egypt Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, Egypt Poultry Production Department, Faculty of Agriculture, New Valley University, New Valley, Egypt

    Article Title: Cecropia pachystachya Improves Naproxen-induced Gastric Ulcers in Mice Through its Potent Anti- inflammatory, Antioxidant, and NO Production Activities
    Article Snippet: .. Cytokine levels Interleukin (IL)-6 and tumor necrosis factor (TNF)-α levels in the gastric tissues were quanti ed by ELISA using the DuoSet Kit (R&D Systems, USA). ..

    Article Title: The soluble receptor for advanced glycation end products is potentially predictive of pulmonary arterial hypertension in systemic sclerosis
    Article Snippet: .. HMGB1 was quantified using a commercial ELISA kit (TECAN, the Netherlands) while sRAGE was measured using a Duoset kit (R&D systems, United Kingdom), according to manufacturers’ instructions. .. High-performance ELISA buffer (Sanquin, the Netherlands) was used during serum incubation to prevent non-specific reactions.

    Article Title: Endometrial stromal cell miR-19b-3p release is reduced during decidualization implying a role in decidual-trophoblast cross-talk.
    Article Snippet: .. PRL secretion by decidualized hESF (culture media collected on days 3 and 14) was quantified by ELISA as per the manufacturer’s instructions (DuoSet kit #DY682, R&D systems) (23). .. Decidual culture media: RNA was isolated from 200uL culture media collected on Day 3 and Day 14 of culture and media only Frontiers in Endocrinology 03 control using the RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions. hESF & HTR8/Svneo cells, endometrial and decidual tissue: RNA extraction was performed as previously described using Tri Reagent according to the manufacturer's instructions (Sigma-Aldrich, Merck).

    Concentration Assay:

    Article Title: The association between corneal immune cell dynamics and comfort in silicone hydrogel contact lens wearers.
    Article Snippet: .. The concentration of MMP-9 and TIMP-1 was determined using the R&D Systems Inc DuoSet kit (Minneapolis, MN, USA) (MMP-9 catalog No: DY911; TIMP-1 catalog N◦: DY970). ..



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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Image Search Results


    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Journal: Chembiochem

    Article Title: Functional Heterogeneity of the Autism Spectrum Disorder‐Associated Gut Microbial Ecosystem Revealed by Fecal Lipopolysaccharides and Bacterial Proteomic Profiling

    doi: 10.1002/cbic.70557

    Figure Lengend Snippet: Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Article Snippet: Cytokine levels (pg/mL) in HMC3 supernatants were measured using DuoSet ELISA kits (R&D Systems, Minneapolis, MN, USA) for human IL‐8 (#DY208) and IL‐6 detection (#DY206).

    Techniques: Activation Assay, MTT Assay, Negative Control, Enzyme-linked Immunosorbent Assay, Comparison